Abstract Zeyart AL-Arbaeen is the largest annual religious gathering in the world, with millions of Shia Muslims visiting Karbala, Iraq, every year. This study aims to investigate the impact of environmental collaboration on sustainable Islamic tourism development, Taking Zeyart AL-Arbaeen as a case study. The research employed a mixedmethod approach, involving both qualitative and quantitative data collection methods. Data were collected from both primary and secondary sources, including interviews, surveys, and literature reviews. The findings indicate that environmental collaboration can positively impact sustainable Islamic tourism development by promoting sustainable practices, reducing environmental degradation, enhancing socio-cultural awareness, and fostering economic growth. The study also recommends that the Iraqi government, in collaboration with religious leaders and other stakeholders, develop policies and strategies to ensure the sustainability of Zeyart AL-Arbaeen